Chapter 643 Finding Phages in the Septic Tank

Facing worker Lu Wenjie's most severely burned right lower limb was emitting a stench, the doctors knew that the condition was getting worse just by smelling it, and the bacteria were constantly increasing, not to mention the blood inflammatory indicators were flying up.

It's okay if it's limited to the right lower limb, but the bacteria produced will enter the blood and then flow to all parts of the body. This is sepsis, or sepsis.

It is precisely this that burn patients are most afraid of, because they will die.

Chen Qi, Guo Yuanhang, Zhu Huoyan, Luo Yuyang, Sun Chengzhi from the Orthopedics Department, Shen Zhemin from the General Surgery Department, and other surgeons looked at the patient's right leg and worried.

Outside the ward, Professor Peng Yongyan, Professor Ni Zhihua, Professor Zhu Lianggong, Professor Wang Qizheng, and Professor An Yongchun, several top domestic experts, accompanied by Lan Lijuan, were also conducting internal medicine consultations.

I have to say that Lu Wenjie and several other burn patients are extremely lucky that this batch of uninvited experts are here without any red envelopes.

However, in order to verify the clinical level of Yuezhong Hospital and to see if they have any "unique tricks", the expert group made up their minds to remain neutral and not to express their opinions or point fingers.

Professor Ni Zhihua, in particular, was a bit conflicted in his heart. On the one hand, he didn't want the patient to die, and on the other hand, he didn't want the Yuezhong Hospital to cure the patient.

In the ward.

After checking with gloves on, Shen Zhemin smacked his lips:

"I think amputation is still necessary. You can see that from here to here, there is edema and necrotic fluid oozing. In addition, the patient is now having a high fever and confusion. This right lower limb is really impossible to keep."

As soon as the words came out, the little doctors behind all nodded in agreement.

Surgeons have simple thinking, are they sick? cut! Let's cut it clean.

But as a "famous doctor", Chen Qi obviously doesn't want to give up hope. After all, theoretically, as long as the inflammation is under control, the patient's right lower limb can still be preserved.

Then the question goes back to the original point, how to control inflammation, or **** bacteria.

Zhu Huoyan coughed lightly at this time, attracting everyone's attention:

"Last night I looked through a lot of literature, and I saw a bactericidal solution that our country had introduced from the Soviet Union in the 1950s and 1960s. Guo Shuji, do you remember a kind called 'bacteriophage', which was also used in our hospital back then? used."

Phage?

In the ward, a few old doctors uttered oh, obviously remembering something.

The young doctors, including Chen Qi, were at a loss, and the stupidity in their eyes became more and more obvious, because they had never learned this medical concept.

"Mr. Zhu, what is a bacteriophage? I, I am also a person who has read a lot of literature, why haven't I heard of it?"

Old Guo laughed loudly when he heard this: "Haha, Chen Qi, it's finally time for you to not understand, Lao Zhu, teach this kid a lesson!"

Outside the ward, Lan Lijuan, Ma Xiaona and others were also confused when they heard "bacteriophage", and then their minds turned quickly, thinking about whether they had learned it before.

On the contrary, several professors also laughed.

Professor Peng joked: "Why, you little comrades don't know about phages?"

Lan Lijuan blushed. She was still a member of the International Medical Association and the director of the department. She had never even heard of a medical concept. This shame was not only her own face, but also the face of Yuezhong Hospital.

Different from Chen Qi's hard-talking, Lan Lijuan is a traditional and typical good student who asks for advice when she doesn't understand.

"Professors, I really don't know about this bacteriophage. You also know that our primary education is only technical secondary school, and the later undergraduate and graduate students are recommended all the way. They didn't take classes well, so there is a lack of study. The professor taught us."

Wang Qi is an old professor of Zhejiang Medical University and has the most say:

"Comrade Xiaolan doesn't need to blush, because this bacteriophage is not included in undergraduate and graduate teaching textbooks, because it is a treatment plan that has been eliminated."

Professor An Yongchun of Xiangya Hospital also expressed emotion:

"Yeah, back then we were poor at home and couldn't even produce penicillin, and foreign countries imposed an embargo on us, so many antibiotics couldn't come in, and we had to smuggle some from the south. Our country is so big, this smuggling is not enough Woolen cloth?

Without medicine, we can't heal patients, especially those soldiers with war wounds, so later we developed a bacteriophage treatment plan, which is also a helpless alternative. Later, with more and more antibacterial drugs , phage therapy has also been phased out. "

In the ward, Zhu Huoyan was also teaching Chen Qi:

"The phage we are talking about is a general term for viruses that infect microorganisms such as bacteria, fungi, algae, actinomycetes, or spirochetes. They can cause the lysis of host bacteria. Phages have some characteristics of viruses. , containing only a single nucleic acid."

Chen Qi smiled awkwardly: "I don't understand."

Zhu Huoyan has a good temper. If it was Lao Guo, he would curse again. He continued to explain patiently:

"A phage can be understood as a microorganism that eats bacteria, or a virus, and does not directly infect animals."

Chen Qi was stunned when he heard it, did you:

"Nani? Such a powerful thing eats bacteria directly, so why haven't I heard of it? What is the principle of eating bacteria?"

Outside the ward, Professor Wang Qizheng is teaching Lan Lijuan and the others a lesson

Traditional antibiotics are bacteriostatic or bactericidal, and the main mechanism is: inhibiting bacterial cell wall synthesis, enhancing bacterial cell membrane permeability, interfering with bacterial protein synthesis, and inhibiting bacterial nucleic acid replication and transcription.

The host range of bacteriophages is generally narrower than that of antibiotics. A phage is generally only effective against a specific bacterium, or a specific strain of a bacterium, helping patients attack bacteria that cause specific diseases.

They use the bacterium's resources to produce offspring phages, and when the bacterium's resources are exhausted, they screw up the whole bacterium, which not only kills the bacterium, but also releases more phages, which go on to find the next dying batch bacteria.

Compared with antibiotics, the benefits of bacteriophages are obvious. Antibiotics are less targeted and tend to kill probiotics in the body indiscriminately. However, phages are unique in their precise elimination of super bacteria and their harmless effect on the human body. "

Lan Lijuan was surprised: "Since bacteriophages are so useful, why were they eliminated later?"

Professor Peng smiled lightly:

"The main thing is that it is unstable. Not only is the curative effect unstable, but the source of obtaining it is also unstable. It is definitely not as convenient, quick and effective as antibiotics. So when our country can produce antibacterial drugs, it will be quickly eliminated."

Professor Wang also joked:

"Think about it. In the 1950s and 1960s, those who had money to treat diseases were either rich or expensive. Anyway, ordinary people definitely couldn't afford injections. It turned out that they would give you a shot of the virus when you were treated. Good guy, How can this patient accept it?"

Hehehe, the atmosphere outside the ward is relaxed.

In the ward, Chen Qi finally understood what a phage is:

"I understand, it is now necessary to find a Pseudomonas aeruginosa phage, or Staphylococcus aureus phage, and then inject it into the patient, and then let the phage find the corresponding bacteria to devour, and have a full meal, is this the rationale? "

Zhu Huoyan chuckled:

"It doesn't have to be injected. In fact, we can make our own phage liquid, and then soak the patient's right lower limb in it, let the phage find Pseudomonas aeruginosa, and then eat it, which also plays an anti-infection role."

Chen Qi asked again:

"Then how should this phage be obtained? Is it based on bacterial culture?"

Old Guo frowned at this time, as if thinking of something disgusting:

"It's not difficult to find bacteriophages, but the difficulty is that the dirtier the place, the easier it is to find them, like cesspools, sewers, rotting animal carcasses, etc. It's like finding a needle in a haystack."

At that time, there were many cesspools in public toilets in the city, and most people in the urban area were using toilets or spittoons at home, and very few of them built a flush toilet like the Chen family.

And the excrement in this cesspool is a good thing. People in the countryside will shake the cement boat to transport it to plant vegetables, and often fight over the excrement for this reason.

But these days, neither the sanitation station nor the common people have a strong sense of hygiene, and the public toilets are terribly smelly.

When Chen Qi thought that he was going to find phages in such a disgusting place, he retreated for a while.

"Are you going to look for bacteriophages in the septic tank sewer dump?"

Outside the ward, after hearing the professors' explanation, Lan Lijuan looked at her husband through the glass.

Knowing husband Mo Ruo's wife, Chen Qi can tolerate the rotten smell caused by corpse-smelling bacteria, but he can't bear the stench of defecation.

So Chen's family was the first to install flush toilets on Lu Xun Road, and it's enough to flush once when others go to the toilet.

Asking Chen Qi to look for phages in the cesspool, Lan Lijuan knew that this guy must be unwilling.

But the job of finding bacteria must be done by medical personnel. Ordinary people don't know how to take specimens or how to cultivate phages.

So Dr. Lan, who aspired to be an infectious disease expert, stood up and shouted to Chen Qi inside the intercom:

"Chen Qi, why don't you leave the search for the phage to me, and I'll take Yang Yibing and the others to look for it together?"

Both Lao Guo and Zhu Huoyan looked at Chen Qi with disdain...

Chen Qi coughed a few times, knowing that at this time, as the person in charge of the medical team, a young leading cadre, he must take the lead in imitating, not to mention that even lesbians who are not in the burn surgery department are going to be involved. I'm sorry.

But Chen Qi obviously wouldn't let the Yue Steel Factory go. In order to save their workers, he was asked to be a big director to urinate? It's almost crazy.

"Is Chief Zuo here?"

Medical Department Chief Zuo Liwei responded outside the ward: "I'm here, Dean Chen."

"Old Zuo, please make arrangements. In the afternoon, Dr. Lan and I will lead the team, and the doctors from the laboratory department will also be with us. Then you will pull out all the interns and 15 students from the endoscopy class in our hospital.

Half of them went to public toilets, garbage dumps, and sewers in urban areas to look for phages, and the other time went to open-air cesspit, muddy land, and smelly drains in suburban countryside to look for phages, and told them that they would be subsidized by 20 yuan per person.

Oh, by the way, after the number of people is counted, report to Qi Guomin of the Yue Steel Factory and ask him to pay for it. Grandma, it's not that he said that he will do whatever it takes. We don't need to save money for him. Student subsidies. "

When Zuo Liwei heard this, he nodded again and again: "Alright Dean Chen, I will go with the students this afternoon."

Chen Qi turned his head to look at Lao Guo and Zhu Huoyan, and asked sinisterly, "Where are the two teachers?"

Zhu Huoyan didn't turn his head: "I want to keep an eye on the patient at any time."

Old Guo looked at the ceiling, "Don't count on me, I'm going to the bureau for a meeting in the afternoon."

That afternoon, a total of 60 interns, 15 master students, together with Chen Qi, Lan Lijuan and 13 other teachers, formed 15 excrement-seeking groups of 6 people.

One teacher and five students walked out of the gate of Yuezhong Hospital and walked in all directions with various feces spoons, buckets, glass bottles and other tools.

There are thousands of bacteria in feces, such as Escherichia coli, Enterococcus faecium, Bacillus aerogenes, Clostridium, Shigella, Vibrio parahaemolyticus and so on.

One kind of bacterium corresponds to one kind of phage, so there are thousands of kinds of phages, and now Chen Qi only needs two kinds of phages, Pseudomonas aeruginosa and Staphylococcus aureus.

So it is very difficult to find it, and it needs a lot of manpower, otherwise it will take a few days to find it, and the patient will have to have amputation.

A public toilet in an urban area.

With two cotton **** stuffed into Chen Qi's nose, he was stirring the excrement vigorously with a dung spoon. What kind of poop, toilet paper, menstrual belt, and even the dead cats and dogs' corpses all came up, emitting a strong smell. stench.

"vomit~~vomit~~~"

Chen Qi retched a few times, his whole face was swollen and red.

Beside Zhu Jianwei quickly flattered him: "Mr. Chen, let me come. I come from the countryside. I am not afraid of getting dirty."

Chen Qi waved his hands while retching: "No, I'm a teacher, and I want to lead by example. Hurry up and take a good specimen of this spoonful of feces, and record the time and place."

Jin Meng is a girl from the city. She has never seen such a battle. After the dung was brought up, she accidentally knocked over the bottle, and the dung spilled on her sneakers.

"vomit~~vomit~~~"

She couldn't bear it anymore, jumped away quickly, and then leaned on a tree and began to vomit wildly.

Originally, Chen Qi didn't vomit, but when Jin Meng vomited like this, he couldn't help it anymore. Holding on to the other side of the tree, he also vomited heavily.

vomit~~~

vomit~~~

Zhu Jianwei and Guan Zelong glanced at each other, their eyes full of helplessness, and they had to be two rural students to stand up at the critical moment.

In the evening, the No. 15 excrement-seeking army came back, and the laboratory started working overnight, and finally found the clinically needed bacteriophages in the standard equipment collected in an open-air cesspit in Tingshan Township.

That night, Comrade Chen used the imported shower gel to wash over and over again...

(end of this chapter)