New observations on the natural history of bees.

by Francis Huber.

TRANSLATOR'S PREFACE

The facts contained in this volume are deeply interesting to the Naturalist. They not only elucidate the history of those industrious animals, whose nature is the peculiar subject of investigation, but they present some singular features in physiology which have hitherto been unknown.

The industry of bees has proved a fertile source of admiration in all countries and in every age; and mankind have endeavoured to render it subservient to their gratifications or emolument. Hence innumerable theories, experiments, and observations have ensued, and uncommon patience has been displayed in prosecuting the enquiry. But although many interesting peculiarities have been discovered, they are so much interwoven with errors, that no subject has given birth to more absurdities than investigations into the history of bees: and unfortunately those treatises which are most easily attained, and the most popular, only serve to give such absurdities a wider range, and render it infinitely more difficult to eradicate them. A considerable portion of the following work is devoted to this purpose. The reader will judge of the success which results from the experiments that have been employed.

Perhaps this is not the proper place to bestow an encomium on a treatise from which so much entertainment and instruction will be derived.

However, to testify the estimation in which it is held in other nations, the remarks upon it by the French philosopher Sue, may be quoted, 'The observations are so consistent, and the consequences seem so just, that while perusing this work, it appears as if we had a.s.sisted the author in each experiment, and pursued it with equal zeal and interest. Let us invite the admirers of nature to read these observations; few are equal to them in excellence, or so faithfully describe the nature, the habits, and inclinations of the insects of which they treat.'

It is a remarkable circ.u.mstance that the author laboured under a defect in the organs of vision, which obliged him to employ an a.s.sistant in his experiments. Thus these discoveries may be said to acquire double authority. But independent of this the experiments are so judiciously adapted to the purposes in view, and the conclusions so strictly logical, that there is evidently very little room for error. The talents of _Francis Burnens_, this philosophic a.s.sistant, had long been devoted to the service of the author, who, after being many successive years in this manner aided in his researches, was at last deprived of him by some unfortunate accident.

Whether the author has prosecuted his investigation farther does not appear, as no other production of his pen is known in this island.

It is vain to attempt a translation of any work without being to a certain degree skilled in the subject of which it treats. Some parts of the original of the following treatise, it must be acknowledged, are so confused, and some so minute, that it is extremely difficult to give an exact interpretation. But the general tenor, though not elegant, is plain and perspicuous; and such has it been here retained.

LETTER I.

_ON THE IMPREGNATION OF THE QUEEN BEE._

SIR,

When I had the honour at Genthod of giving you an account of my princ.i.p.al experiments on bees, you desired me to transmit a written detail, that you might consider them with greater attention. I hasten, therefore, to extract the following observations from my journal.--As nothing can be more flattering to me than the interest you take in my researches, permit me to remind you of your promise to suggest new experiments{A}.

After having long studied bees in gla.s.s hives constructed on M. de Reaumur's principle, you have found the form unfavourable to an observer. The hives being too wide, two parallel combs were made by the bees, consequently whatever pa.s.sed between them escaped observation.

From this inconvenience, which I have experienced, you recommended much thinner hives to naturalists, where the panes should be so near each other, that only a single row of combs could be erected between them. I have followed your admonitions, Sir, and provided hives only eighteen lines in width, in which I have found no difficulty to establish swarms.

However, bees must not be entrusted with the charge of constructing a single comb: Nature has taught them to make parallel ones, which is a law they never derogate from, unless when constrained by some particular arrangement. Therefore, if left to themselves in these thin hives, as they cannot form two combs parallel to the plane of the hive, they will form several small ones perpendicular to it, and, in that case, all is equally lost to the observer. Thus it became essential previously to arrange the position of the combs. I forced the bees to build them perpendicular to the horizon, and so that the lateral surfaces were three or four lines from the panes of the hive. This distance allows the bees sufficient liberty, but prevents them from collecting in too large cl.u.s.ters on the surface of the comb. By such precautions, bees are easily established in very thin hives. There they pursue their labours with the same a.s.siduity and regularity; and, every cell being exposed, none of their motions can be concealed.

It is true, that by compelling these insects to a habitation where they could construct only a single row of combs, I had, in a certain measure, changed their natural situation, and this circ.u.mstance might possibly have affected their instinct. Therefore, to obviate every objection, I invented a kind of hives, which, without losing the advantages of those very thin, at the same time approached the figure of common hives where bees form several rows of combs.

I took several small fir boxes, a foot square and fifteen lines wide, and joined them together by hinges, so that they could be opened and shut like the leaves of a book{B}. When using a hive of this description, we took care to fix a comb in each frame, and then introduced all the bees necessary for each particular experiment. By opening the different divisions successively, we daily inspected both surfaces of every comb. There was not a single cell where we could not distinctly see what pa.s.sed at all times, nor a single bee, I may almost say, with which we were not particularly acquainted. Indeed, this construction is nothing more than the union of several very flat hives which may be separated. Bees, in such habitations, must not be visited before their combs are securely fixed in the frames, otherwise, by falling out, they may kill or hurt them, as also irritate them to that degree that the observer cannot escape stinging, which is always painful, and sometimes dangerous: but they soon become accustomed to their situation, and in some measure tamed by it; and, in three days, we may begin to operate on the hive, to open it, remove part of the combs, and subst.i.tute others, without the bees exhibiting too formidable symptoms of displeasure. You will remember, Sir, that on visiting my retreat, I shewed you a hive of this kind that had been a long time in experiment, and how much you were surprised that the bees so quietly allowed us to open it.

In these hives, I have repeated all my observations, and obtained exactly the same results as in the thinnest. Thus, I think, already to have obviated any objections that may arise concerning the supposed inconvenience of flat hives. Besides, I cannot regret the repet.i.tion of my labours; by going over the same course several times, I am much more certain of having avoided error; and it also appears, that some advantages are found in these which may be called _Book_ or _Leaf-hives_, as they prove extremely useful in the economical treatment of bees, which shall afterwards be detailed.

I now come to the particular object of this letter, the fecundation of the queen bee; and I shall, in a few words, examine the different opinions of naturalists on this singular problem. Next I shall state the most remarkable observations which their conjectures have induced me to make, and then describe the new experiments by which I think I have solved the problem{C}.

Swammerdam, who studied bees with unremitting attention, and who never could see a real copulation between a drone and a queen, was satisfied that copulation was unnecessary for fecundation of the eggs: but having remarked that, at certain times, the drones exhaled a very strong odour, he thought this odour was an emanation of the _aura seminalis_, or the _aura seminalis_ itself, which operated fecundation by penetrating the body of the female. His conjecture was confirmed on dissecting the male organs of generation; for he was so much struck with the disproportion between them and those of the female, that he did not believe copulation possible. His opinion, concerning the influence of the odour, had this farther advantage, that it afforded a good reason for the prodigious number of the males. There are frequently fifteen hundred or two thousand in a hive; and, according to Swammerdam, it is necessary they should be numerous, that the emanation proceeding from them may have an intensity or energy sufficient to effect impregnation.

Though M. de Reaumur has refuted this hypothesis by just and conclusive reasoning, he has failed to make the sole experiment that could support or overturn it. This was to confine all the drones of a hive in a tin case, perforated with minute holes, which might allow the emanation of the odour to escape, but prevent the organs of generation from pa.s.sing through. Then, this case should have been placed in a hive well inhabited, but completely deprived of males, both of large and small size, and the consequence attended to. It is evident, had the queen laid eggs after matters were thus disposed, that Swammerdam's hypothesis would have acquired probability; and on the contrary it would have been confuted had she produced no eggs, or only sterile ones. However the experiment has been made by us, and the queen remained barren; therefore, it is undoubted, that the emanation of the odour of the males does not impregnate bees.

M. de Reaumur was of a different opinion. He thought that the queen's fecundation followed actual copulation. He confined several drones in a gla.s.s vessel along with a virgin queen: he saw the female make many advances to the males; but, unable to observe any union so intimate that it could be denominated copulation, he leaves the question undecided. We have repeated this experiment: we have frequently confined virgin queens with drones of all ages: we have done so at every season, and witnessed all their advances and solicitations to the males: we have even believed we saw a kind of union between them, but so short and imperfect that it was unlikely to effect impregnation. Yet, to neglect nothing, we confined the virgin queen, that had suffered the approaches of the male, to her hive. During a month that her imprisonment continued, she did not lay a single egg; therefore, these momentary junctions do not accomplish fecundation.

In the _Contemplation de la Nature_, you have cited the observations of the English naturalist Mr Debraw. They appear correct, and at last to elucidate the mystery. Favoured by chance, the observer one day perceived at the bottom of cells containing eggs, a whitish fluid, apparently spermatic, at least, very different from the substance or jelly which bees commonly collect around their new hatched worms.

Solicitous to learn its origin, and conjecturing that it might be the male prolific fluid, he began to watch the motions of every drone in the hive, on purpose to seize the moment when they would bedew the eggs. He a.s.sures us, that he saw several insinuate the posterior part of the body into the cells, and there deposit the fluid. After frequent repet.i.tion of the first, he entered on a long series of experiments. He confined a number of workers in gla.s.s bells along with a queen and several males.

They were supplied with pieces of comb containing honey, but no brood.

He saw the queen lay eggs, which were bedewed by the males, and from which larvae were hatched, consequently, he could not hesitate advancing as a fact demonstrated, that male bees fecundate the queen's eggs in the manner of frogs and fishes, that is, after they are produced.

There was something very specious in this explanation: the experiments on which it was founded seemed correct; and it afforded a satisfactory reason for the prodigious number of males in a hive. At the same time, the author had neglected to answer one strong objection. Larvae appear when there are no drones. From the month of September until April, hives are generally dest.i.tute of males, yet, notwithstanding their absence, the queen then lays fertile eggs. Thus, the prolific fluid cannot be required to impregnate them, unless we can suppose that it is necessary at a certain time of the year, while at every other season it is useless.

To discover the truth amidst these facts apparently so contradictory, I wished to repeat Mr Debraw's experiments, and to observe more precaution than he himself had done. First, I sought for the fluid, which he supposes the seminal, in cells containing eggs. Several were actually found with that appearance; and, during the first days of observation, neither my a.s.sistant nor myself doubted the reality of the discovery.

But we afterwards found it an illusion arising from the reflection of the light, for nothing like a fluid was visible, except when the solar rays reached the bottom of the cells. Fragments of the cocc.o.o.ns of worms, successively hatched, commonly cover the bottom; and, as they are shining, it may easily be conceived that, when much illuminated, an illusory effect results from the light. We proved it by the strictest examination, for no vestiges of a fluid were perceptible when the cells were detached and cut asunder.

Though the first observation inspired us with some distrust of Mr Debraw's discovery, we repeated his other experiments with the utmost care. On the 6. of August 1787, we immersed a hive, and, with scrupulous attention, examined the whole bees while in the bath. We ascertained that there was no male, either large or small; and having examined all the combs, we found neither male nymph, nor worm. When the bees were dry, we replaced them all, along with the queen, in their habitation, and transported them into my cabinet. They were allowed full liberty; therefore, they flew about, and made their usual collections; but, it being necessary that no male should enter the hive during the experiment, a gla.s.s tube was adapted to the entrance, of such dimensions that two bees only could pa.s.s at once; and we watched the tube attentively during the four or five days that the experiment continued.

We should have instantly observed and removed any male that appeared, that the result of the experiment might be undisturbed, and I can positively affirm that not one was seen. However, from the first day, which was the sixth of August, the queen deposited fourteen eggs in the workers cells; and all these were hatched on the tenth of the same month.

This experiment is decisive, since the eggs laid by the queen of a hive where there were no males, and where it was impossible one could be introduced, since these eggs, I say, were fertile, it becomes indubitable that the fluid of the males is not required for their exclusion.

Though it did not appear that any reasonable objection could be started against this conclusion, yet, as I had been accustomed in all my experiments to seek for the most trifling difficulties that could arise, I conceived that Mr Debraw's partisans might maintain, that the bees, deprived of drones, perhaps would search for those in other hives, and carry the fecundative fluid to their own habitations for depositing it on the eggs.

It was easy to appreciate the force of this objection, for all that was necessary was a repet.i.tion of the former experiments, and to confine the bees so closely to their hives that none could possibly escape. You very well know, Sir, that these animals can live three or four months confined in a hive well stored with honey and wax, and if apertures are left for circulation of the air. This experiment was made on the tenth of August; and I ascertained, by means of immersion, that no male was present. The bees were confined four days in the closest manner, and then I found forty young larvae.

I extended the precautions so far as to immerse this hive a second time, to a.s.sure myself that no male had escaped my researches. Each of the bees was separately examined, and none was there that did not display its sting. The coincidence of this experiment with the other, proved that the eggs were not externally fecundated.

In terminating the confutation of Mr Debraw's opinion, I have only to explain what led him into error; and that was, his using queens whose history he was unacquainted with from their origin. When he observed the eggs produced by a queen, confined along with males, were fertile, he thence concluded that they had been bedewed by the prolific fluid in the cells: but to render his conclusion just, he should first have ascertained that the female never had copulated, and this he neglected.

The truth is, that, without knowing it, he had used, in his experiments, a queen after she had commerce with the male. Had he taken a virgin queen the moment she came from the royal cell, and confined her along with drones in his vessels, the result would have been opposite; for, even amidst a seraglio of males, this young queen would never have laid, as I shall afterwards prove.

The Lusatian observers, and M. Hattorf in particular, thought the queen was fecundated by herself, without concourse with the males. I shall here give an abstract of the experiment on which that opinion is founded.{D}

M. Hattorf took a queen whose virginity he could not doubt. He excluded all the males both of the large and small species, and, in several days, he found both eggs and worms. He a.s.serts that there were no drones in the hive, during the course of the experiment; but although they were absent, the queen laid eggs, from which came worms: whence he considers she is impregnated by herself.

Reflecting on this experiment, I do not find it sufficiently accurate.

Males pa.s.s with great facility from hive to hive; and M. Hattorf took no precaution that none was introduced into his. He says, indeed, there was no male, but is silent respecting the means he adopted to prove the fact. Though he might be satisfied of no large drone being there, still a small one might have escaped his vigilance, and fecundated the queen.

With a view to clear up the doubt, I resolved to repeat his experiment, in the manner described, and without greater care or precaution.

I put a virgin queen into a hive, from which all the males were excluded, but the bees left at perfect liberty. For several days I visited the hive, and found new hatched worms in it. Here then is the same result as M. Hattorf obtained? But before deducing the same consequence from it, we had to ascertain beyond dispute that no male had entered the hive. Thus, it was necessary to immerse the bees, and examine each separately. By this operation, we actually found four small males. Therefore, to render the experiment decisive, not only was it requisite to remove all the drones, but also, by some infallible method, to prevent any from being introduced, which the German naturalist had neglected.

I prepared to repair this omission, by putting a virgin queen into a hive, from which the whole males were carefully removed; and to be physically certain that none should enter, a gla.s.s tube was adapted at the entrance of such dimensions that the working bees could freely pa.s.s and repa.s.s, but too narrow for the smallest male. Matters continued thus for thirty days, the workers departing and returning performed their usual labours: but the queen remained sterile. At the expiration of this time, her belly was equally slender as at the moment of her origin. I repeated the experiment several times, and always with the same consequence.

Therefore, as a queen, rigorously separated from all commerce with the male, remains sterile, it is evident she cannot impregnate herself, and M. Hattorf's opinion is ill-founded.

Hitherto, by endeavouring to confute or verify the conjectures of all the authors who had preceded me, by new experiments, I acquired the knowledge of new facts, but these were apparently so contradictory as to render the solution of the problem still more difficult. While examining Mr. Debraw's hypothesis, I confined a queen in a hive, from which all the drones were removed; the queen nevertheless was fertile.

When considering the opinion of M. Hattorf on the contrary, I put a queen, of whose virginity I was perfectly satisfied, in the same situation, she remained sterile.

Embarra.s.sed by so many difficulties, I was on the point of abandoning the subject of my researches, when at length by more attentive reflection, I thought these contradictions might arise from experiments made indifferently on virgin queens, and on those with whose history I was not acquainted from the origin, and which had perhaps been impregnated unknown to me. Impressed with this idea, I undertook a new method of observation not on queens fortuitously taken from the hive, but on females decidedly in a virgin state, and whose history I knew from the instant they left the cell.

From a very great number of hives, I removed all the virgin females, and subst.i.tuted for each a queen taken at the moment of her birth. The hives were then divided into two cla.s.ses. From the first, I took the whole males both large and small, and adapted a gla.s.s tube at the entrance, so narrow, that no drone could pa.s.s, but large enough for the free pa.s.sage of the common bees. In the hives of the second cla.s.s, I left all the drones belonging to them, and even introduced more; and to prevent them from escaping, a gla.s.s tube, also too narrow for the males, was adapted to the entrance of these hives.

For more than a month, I carefully watched this experiment, made on a large scale; but much to my surprise, all the queens remained sterile.

Thus it was proved, that queens confined in a hive would continue barren though amidst a seraglio of males.

This result induced me to suspect that the females could not be fecundated in the interior of the hive, and that it was necessary for them to leave it for receiving the approaches of the male. To ascertain the fact was easy, by a direct experiment; and as the point is important, I shall relate in detail what was done by my secretary and myself on the 29. June 1788.